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In Vitro Observation of Cholesterol and Glucose Contents in Amphistomes: Paramphistomum Cervi (Zeder1790) Fischoeder1901

Surender Reddy K, Bikshapathi V

 In Vitro Observation of Cholesterol and Glucose Contents in Amphistomes: Paramphistomum Cervi (Zeder1790) Fischoeder1901

The studies of in vitro isolation and the starvation on the cholesterol contents were investigated in Paraphistomum cervi. The cholesterol contents in the body of the parasite slightly decreased when glucose was available (0.071-control, 0.070 (30 min), 0.069 (1hr), 0.067 (2 hr), 0.063 (3hr), 0.060 (4hr), 0.058 (6hr) and 0.052 mg / 100 mg (8 hr). At the end of treatment period (8 hr) the cholesterol contents decreased to 26.76%. When glucose was not available cholesterol contents showed a significant (P<0.05) decrease only up to 8 hours (0.071) for control was 0.068 (30min), 0.066 (1hr), 0.063 (2hr) ,0.061 (3hr), 0.058 (4hr), 056 (6hr), 0.040 (8hr). At the end, the decrease was 43.66%. The body cholesterol content was decreased due to starvation, which might have been used for the release of energy..