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Polyphenolics from Black Spanish Red Wine (Viti's "aestivalis") have Cytotoxic Activity in Colon Cancer Cells and Repress Prooncogenic microRNA-27a

Del Follo-Martinez A, Banerjee N, Li X, Talcott S, Safe S and Mertens-Talcott S

Polyphenolics from Black Spanish Red Wine (Vitis �aestivalis�) have Cytotoxic Activity in Colon Cancer Cells and Repress Prooncogenic microRNA-27a

The anticancer activity of polyphenolics from Black Spanish wine (Vitis "aestivalis"), was investigated in HT-29 colorectal adenocarcinoma cells. Wine polyphenolics significantly inhibited cell proliferation from 18.7 - 300 μg gallic acid equivalents (GAE)/mL over a period of 24h with an IC50 value of 258 μg GAE/mL.Polyphenolics also decreased the generation of reactive oxygen species (ROS) and increased the antioxidant capacity as determined by the oxygen radical absorbance capacity assay (ORAC). The growth inhibitory effects of the polyphenolics were accompanied by an induction of cleavage cleaved caspase-3. Polyphenolic treatment decreased the expression of specifity protein (Sp) transcription factors Sp1, Sp3, and Sp4 mRNA and protein expression. Furthermore, these responses were accompanied by decreased expression of the Sp-dependent survival gene survivin. The wine polyphenolics also decreased the expression of microRNA-27a (miR-27a) and induced zinc finger protein ZBTB10 (anSp-repressor) mRNA which is consistent with previous study. This was confirmed by transfection of colon cells with the specific mimic for miR-27a and wine polyphenolics partially reversed the effects of mimic.